• Title: Sending blood samples
• List item:
  • List title: Book shipping, arrange a courier pick-up before drawing blood, List content:

    Ideally, blood samples should arrive within 24 hours after being drawn. 48 hours is the maximum. Collection by/drop off to courier should be booked the day before the blood is drawn, so the sample can be sent as soon as possible. Depending on your location, FedEx or DHL are the only recommended couriers.

  • List title: Avoid steroids, List content:

    Avoid taking steroids or immuno suppressant drugs as these may affect the test results. Please always check with your healthcare practitioner before stopping medication. Patients suffering from widespread systemic infection should also make this clear to the laboratory ahead of testing as this may make the test results more difficult to evaluate. A list of medication which may affect testing is HERE

  • List title: Send the right amount of blood, List content:

    MELISA needs at least 3.6ml of blood for each metal tested, as well as for testing positive control and several negative controls. We recommend sending the following amount of tubes:

    For 1-3 metals, use 2 x 9 ml tubes or 4 x 4.5 ml tubes
    For 4-6 metals, use 4 x 9 ml tubes or 10 x 4.5 ml tubes
    For 7-10 metals, use 5 x 9 ml tubes or 10 x 4.5 ml tubes
    For 11 to 15 metals, use 6 x 9 ml tubes or 12 x 4.5 ml tubes
    For 16 to 20 metals, use 7 x 9 ml tubes or 14 x 4.5 ml tubes

    If you have a known disease which causes a low lymphocyte count, please send an extra tube. A smaller volume may be sent for children, as they have a denser lymphocyte count. Please check with the laboratory for more details.

  • List title: Use citrate tubes, List content:

    Samples for MELISA testing must be sent in citrate tubes which can be provided by MELISA. These are Becton-Dickinson or Greiner Vacutainer tubes containing sodium citrate, with a sterile interior and a light blue lid. Kits are usually sent from your nearest laboratory or can be available at the clinics listed on this site.

  • List title: Do not centrifuge, do not chill, List content:

    The sodium citrate tubes SHOULD NOT be spun after phlebotomy. They will be centrifuged when they arrive at the laboratory.
    Samples should be sent at room temperature; chilled blood cannot be used in MELISA testing.

  • List title: Include a request form, List content:

    Include a request form clearly stating which panels/substances are required for testing. Every laboratory will have its own test order form.

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• Title: Medication to avoid
• Subtitle: Melisa
• List item:
  • List title: IMMUNOSUPPRESSANT MEDICATION, List content:

    MELISA measures immune response so any drug that suppresses this response may affect results. This is not an exhaustive list and patients should check with their health care professional to see if any of their current medication has an immune suppressant effect. Patients must speak with their healthcare professional before stopping any prescribed medication.

    In certain cases, where it is not possible to cease medication we have incidences of patients with successful test results. It may be useful to send extra blood (1-2 tubes) to ensure that sufficient lymphocytes can be recovered – please consult with MELISA in advance. A reduced fee may be charged if the results are “non-evaluable” due to reduced immune reactivity, as the test will have been set up as normal.

  • List title: Strong effect – avoid ideally for 3 weeks prior to testing, List content:
    • Prednisone/ Prednisolone
    • Dupixent
    • Deltasone
    • Orasone
    • Azathioprine and DMRDs
    • Cortisol
    • Dexamethasone
    • Methotrexate
    • Anti malarials
    • Cosentyx
    • Cyclosporine and Tacrolimus
    • Neoral
    • Sandimmune
    • SangCya
    • Opiods – Long term use at high levels
    • Oral hydrocortisone
    • Methylprednisolone
    • Triamcinolone
    • Hydroxychloroquine (Plaquenil)
  • List title: Possible Mild effect – if possible avoid at least the morning of the blood draw, List content:
    • Anti depressants
    • Anti histamines
    • THC – medical marijuana
    • Finasteride
    • NSAIDs

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